http://rdf.ncbi.nlm.nih.gov/pubchem/patent/JP-H07241189-A

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assignee http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_3c88708de35cf623e91e428e4936a614
classificationIPCInventive http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-04
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N5-07
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N5-09
filingDate 1994-03-04-04:00^^<http://www.w3.org/2001/XMLSchema#date>
inventor http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_882c822056afc9b401ad466ef38b327a
publicationDate 1995-09-19-04:00^^<http://www.w3.org/2001/XMLSchema#date>
publicationNumber JP-H07241189-A
titleOfInvention Cancer cell culture method
abstract (57) [Summary] [Purpose] A cancer cell collected from a cancer tissue contains normal cells, but a culture method that can suppress the growth of normal cells and grow only cancer cells is provided. The purpose is to provide. [Structure] The cell line Cx is cultured in the culture medium 2 of the dish 1, and a thin film of this cell line Cx is placed on the bottom surface of the dish 1. Generate layers. Next, the culture solution 2 is removed, and a 3% solution 3 of glutar aldehyde, which is a cell fixing solution, is placed in the dish 1 to fix the established cell line Cx to the bottom surface of the dish 1. Next, replace culture medium 2 with glutar aldehyde and dish 1 The cells collected from the cancer cells are placed in this culture medium 2. Then, the normal cells C2 having the contact blocking ability contained in the cancer tissue do not grow by contacting the thin film, and only the cancer cells C1 having no contact blocking ability grow.
priorityDate 1994-03-04-04:00^^<http://www.w3.org/2001/XMLSchema#date>
type http://data.epo.org/linked-data/def/patent/Publication

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Total number of triples: 31.