Predicate |
Object |
assignee |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_49f04b1dc6b9fcc2d8b159c5ebee364b |
classificationCPCAdditional |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/B01J2219-00626 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/B01J2219-00605 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/B01J2219-00612 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/B01J2219-00702 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/B01J2219-00722 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/B01J2219-00659 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C40B40-06 |
classificationCPCInventive |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-6869 |
classificationIPCAdditional |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C40B40-06 |
classificationIPCInventive |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G01N21-78 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-09 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G01N33-50 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-68 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-66 |
filingDate |
1994-01-26-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
inventor |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_e2df3b6516f660a0cf895701d9dd5e09 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_b9f525a4788c8df40f7342971164ffd4 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_9b1d2ff31e6c9fedb3fb55c41b703724 |
publicationDate |
1995-08-08-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
publicationNumber |
JP-H07203998-A |
titleOfInvention |
Nucleic acid base sequencing method |
abstract |
(57) [Summary] (Modified) [Objective] To provide a method for determining a nucleotide sequence of a nucleic acid in a short time. [Structure] An oligonucleotide containing a sequence of all k bases is immobilized as a primer in each column. And In this way, an unknown single-stranded test nucleic acid is added to each column of the capillary plate CA in which the primer is fixed in each column to hybridize the primer in each column with the test nucleic acid, and then to each column. 4 kinds of dNTPs are simultaneously added to the solution using a DNA polymerase as a catalyst. As a result, in the 5 ′ to 3 ′ direction of the primer, a base complementary to each base of the test nucleic acid hybridizes with the base of the test nucleic acid, and a unidirectional extension reaction of the primer occurs. By measuring the amount of extension, the bound primer and the binding position between the primer and the test nucleic acid are identified. |
isCitedBy |
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-6376184-B1 http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-6841128-B2 http://rdf.ncbi.nlm.nih.gov/pubchem/patent/KR-20010097731-A http://rdf.ncbi.nlm.nih.gov/pubchem/patent/JP-2008022823-A http://rdf.ncbi.nlm.nih.gov/pubchem/patent/JP-H10337173-A |
priorityDate |
1994-01-26-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
type |
http://data.epo.org/linked-data/def/patent/Publication |