| abstract |
(57) [Summary] [Object] To provide a mutant Escherichia coli ribonuclease HI that retains enzyme activity and has improved stability. [Structure] Natural type E. coli ribonuclease HI No. 23 The Gly of the 62nd, His of the 62nd, Val of the 74th, Lys of the 95th and Asp of the 134th are A, respectively. Mutant Escherichia coli ribonuclease HI introduced with a mutation to replace with la, Pro, Leu, Gly and His (or Asn) Is an enzyme that retains enzyme activity and has improved stability. |