abstract |
(57) [Summary] (Modified) [Purpose] To provide water-soluble coumarin derivatives. [Structure] Coumarin derivative corresponding to general formula (I), β -A method for producing the coumarin derivative using a ketone ester as a starting material, β-ketone ester as a starting material, and using the coumarin derivative of the general formula (I) as an enzyme substrate in the enzyme preparation process. [In the formula, R 1 is-{OCH (R 8 ) CH 2 } OCH 2 R 7 represents; R 2 to R 6 are H, is a halogen such as, in particular, the linking group R 4 is to enable the introduction of appropriate groups on the coumarin derivative for the purpose of synthesis of an enzyme substrate, for example, -NH -CO-CH (NH 2) -CH 2 -CH (C H 3 ) 2 is desirable. R 7 is OH, Substituents such as NH 2 and COOH, R 8 is H or CH 3 ] |