| abstract |
(57) [Summary] (Modified) [Purpose] Use only carboxypeptidase Y even when a single or multiple amino acid residues are attached to the α-carboxyl side of proline to be amidated. Finally, it provides a method for obtaining a peptide having a C-terminal prolinamide. [Structure] One kind of amino acid residue selected from leucine, isoleucine, valine, phenylalanine and alanine is bonded to C-terminal proline, and then one or two A method for producing a peptide having a prolinamide at the C terminus by using a peptide having at least one amino acid residue bound as a substrate and allowing carboxypeptidase Y to act in the presence of ammonia. However, one or two linked above Excludes proline at one or more amino acid residues. |