| abstract |
(57) [Summary] [Purpose] The surface plasmon resonance is used to quantify specific molecules in a sample solution that show a specific reaction to a molecular recognition functional substance. [Structure] A metal thin film 2, a molecular recognition functional film 5, and a flow path 7 through which an analyte 6 flows are arranged on one surface of a prism 1. The incident light 3 emitted from the white light source 8 is converted into a p-polarized light wave by the polarizer 10, and further collimated by the collimator lens 11. The incident light 3 is incident on the surface of the metal thin film 2 in an angle range where the incident angle is changed, but the surface plasmon is excited at a specific angle to reduce the intensity of the reflected light 4. When the refractive index, the dielectric constant, etc. of the sample 6 to be analyzed changes due to the action of the molecular recognition functional substance fixed to the molecular recognition functional film 5, the incident angle (resonance angle) at which the surface plasmon is excited changes. By detecting this change, the concentration of the specific substance in the sample 6 to be analyzed can be calculated. |