http://rdf.ncbi.nlm.nih.gov/pubchem/patent/JP-H06319599-A
Outgoing Links
| Predicate | Object |
|---|---|
| assignee | http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_41686a979f961bce6a9855b4b409a5b8 |
| classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-6848 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-6844 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-6844 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-09 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-68 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-6848 |
| filingDate | 1994-05-11-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor | http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_17a51018ab0edd36df011b524df01872 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_e7415e5c51616d60df734ddf8603a9d9 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_e7ef4fee9b3e0cb97f900b31ad1d8589 |
| publicationDate | 1994-11-22-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | JP-H06319599-A |
| titleOfInvention | Decontamination method for nucleic acid amplification reaction |
| abstract | (57) Abstract: A method for inactivating contaminating amplicons in isothermal nucleic acid amplification reactions, such as SDA, Qβ and 3SR, is provided. Construction: dU is incorporated in place of the thymidine (T) amplicon produced by amplification. If these amplicons contaminate subsequent amplification reactions, they are inactivated as templates by treating them with UDG (ie, Cannot be amplified). The inclusion of the UDG inhibitor protein Ugi inactivates UDG during subsequent amplification of a particular target sequence, as isothermal amplification does not involve high temperatures. d The incorporation of U was unexpectedly found to enhance the amplification capacity of SDA compared to the normal SDA reaction. This method is also used to detect UDG activity in a reagent or sample. |
| priorityDate | 1993-05-11-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 113.