| abstract |
(57) [Summary] [Structure] D containing genes that complement defects in host cells In the NA construct, the defect is in the function required for normal cell growth in complex culture medium, and the DNA sequence is α-1- A DNA construct encoding antitrypsin. [Effect] According to the present invention, a DNA construct capable of expressing α-1-antitrypsin and maintained at a high copy number without requiring a special selective culture medium, and a suitable host cell are provided. Growth in such conditions is faster than It results in higher cell densities and reduced production costs. |