http://rdf.ncbi.nlm.nih.gov/pubchem/patent/JP-H06205699-A
Outgoing Links
| Predicate | Object |
|---|---|
| assignee | http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_f5324eae7242827a83eaa2b6d8f5e3f4 http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_f0db6b222cc066a8553326f9a24f53e1 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-40 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-34 |
| filingDate | 1993-01-11-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor | http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_a150cf72bed945ba096feb8772d21386 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_5cf2d2a8f51fe0e1a7e7987da818f48d |
| publicationDate | 1994-07-26-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | JP-H06205699-A |
| titleOfInvention | Method for measuring α-amylase activity and reagent for measuring α-amylase activity |
| abstract | (57) [Summary] [Objective] To provide an α-amylase activity measuring method capable of accurately and simply measuring the true activity of α-amylase using an automatic analyzer, and a reagent for use in this measuring method. To do. [Structure] A substrate containing a maltooligosaccharide whose reducing end is modified with a dye and a sample containing α-amylase in the coexistence of calcium ions are used to optimize the activity of α-amylase. The first step of degrading the substrate by acting with H, α-glucosidase and calcium ion scavenger to the product obtained in the first step, the second step of acting at the activity optimum pH of α-glucosidase, and An α-amylase activity measuring method comprising a third step of colorimetrically quantifying the dye produced in the two steps. A first solution containing a substrate consisting of a dye-modified maltooligosaccharide, a water-soluble calcium salt compound and a buffer solution having an activity optimum pH of α-amylase, and α-glucosidase, Reagent for measuring α-amylase activity comprising a second liquid containing a calcium ion capturing agent and a buffer solution |
| isCitedBy | http://rdf.ncbi.nlm.nih.gov/pubchem/patent/JP-2013068642-A http://rdf.ncbi.nlm.nih.gov/pubchem/patent/JP-2006136882-A |
| priorityDate | 1993-01-11-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 70.