| abstract |
(57) [Summary] [Structure] The cDNA encoding the antibody heavy chain variable region and the cDNA encoding the antibody light chain variable region were obtained from a hybridoma producing a mouse or rat monoclonal antibody that reacts with ganglioside GM 2 , C encoding a heavy chain constant region or a human antibody light chain constant region By inserting them into an expression vector for animal cell having DNA, to construct a humanized antibody expression vector, to obtain a transformant by introducing into an animal cell, the ganglioside GM 2 by culturing the transformant strain A reactive humanized antibody is obtained. [Effect] The humanized antibody of the present invention is less capable of producing an anti-mouse immunoglobulin antibody in the body of a patient than a mouse monoclonal antibody, so that side effects are reduced and the blood half-life is extended. A better effect is expected. |