http://rdf.ncbi.nlm.nih.gov/pubchem/patent/JP-H06153995-A
Outgoing Links
Predicate | Object |
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assignee | http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_c1755eede76ea7e719a3e0d2843cd793 |
classificationIPCAdditional | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12R1-01 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12R1-19 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12R1-63 |
classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-32 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-54 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-66 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N9-02 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N1-21 |
filingDate | 1992-11-26-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
inventor | http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_56615fcb725dd52d4ca2540f683b1245 |
publicationDate | 1994-06-03-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
publicationNumber | JP-H06153995-A |
titleOfInvention | Assay using bacterial hololuciferase |
abstract | (57) [Summary] [Structure] This is a method for measuring NADH or NADPH in a test sample in which the test sample, a luminescent bacterium-derived hololuciferase and an aldehyde are mixed and the light generated is measured. In addition, the sample to be tested, NAD + or NADP + , dehydrogenase specific to the target substance to be measured, phorluciferase derived from a luminescent bacterium and aldehyde are mixed, and the generated light is measured. Measurement of the substrate of dehydrogenase in the sample to be tested Is the law. [Effect] This measurement method has extremely high sensitivity and a wide measurement range. Further, in the assay method of the present invention, the cost for the assay can be reduced because the assay can be performed only with the present hololuciferase without using expensive NHR. Also, NA By combining an enzyme system that produces DH or NADPH, for example, a dehydrogenase specific to a certain in-vivo trace substance, the in-vivo trace substance can be measured with high sensitivity. |
isCitedBy | http://rdf.ncbi.nlm.nih.gov/pubchem/patent/WO-2021167011-A1 |
priorityDate | 1992-11-26-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 1480.