http://rdf.ncbi.nlm.nih.gov/pubchem/patent/JP-H06153953-A
Outgoing Links
Predicate | Object |
---|---|
assignee | http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_438312df8a4ebd3fa8c445da07cb72aa http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_ed79bd3badc72d84863de7cd3516db07 http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_941153fb5433510bda4b86b2262dcb6f |
classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-1096 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-66 |
classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-66 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-09 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-10 |
filingDate | 1993-03-03-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
inventor | http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_378bfd2d515ac68a364394c6595318b9 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_149ec9194225ed8c027b19cc5311f270 |
publicationDate | 1994-06-03-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
publicationNumber | JP-H06153953-A |
titleOfInvention | Method for synthesizing full-length cDNA, method for producing intermediate thereof, and method for producing recombinant vector containing full-length cDNA |
abstract | (57) [Summary] (Modified) [Objective] To selectively synthesize only full-length cDNA containing a sequence starting from the capping site of mRNA to provide a cDNA containing all primary structure information of the protein. [Structure] A step of removing a phosphate group from the 5 ′ end of an uncapped incomplete-length mRNA, a step of removing a cap from a capped end, and a 5 ′ terminal phosphate group having the following formula The expressed ones are ligated with T4 RNA ligase, and a DNA oligonucleotide or DNA-having a selective sequence at the 5'end of full-length mRNA A method for synthesizing full-length cDNA from mRNA via the step of adding an RNA chimeric oligonucleotide. 5'-dN 1 -dN 2 -...- dN m -N 1 -N 2 -...- N n -3 '(wherein dN is any of dAMP, dCMP, dGMP, dTMP Deoxyribonucleotides, N for AMP, CMP, GM A ribonucleotide of either P 1 or UMP, and-represents a phosphate bond. m is an integer of 1 or more, n Represents an integer of 0 or 1 or more. ) |
isCitedBy | http://rdf.ncbi.nlm.nih.gov/pubchem/patent/WO-2004087916-A1 http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-8551739-B2 http://rdf.ncbi.nlm.nih.gov/pubchem/patent/JP-2007075128-A http://rdf.ncbi.nlm.nih.gov/pubchem/patent/WO-9914364-A1 http://rdf.ncbi.nlm.nih.gov/pubchem/patent/JP-2011521625-A |
priorityDate | 1992-09-25-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 442.