http://rdf.ncbi.nlm.nih.gov/pubchem/patent/JP-H06141844-A
Outgoing Links
| Predicate | Object |
|---|---|
| assignee | http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_03d0f52bb4069f8a19d70dcfb697c67a |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12M3-00 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N11-02 |
| filingDate | 1992-11-13-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor | http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_d159f42e68a0f80bd80cff240034cbca http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_e02a3c6c79c861a41df9585b209868e0 |
| publicationDate | 1994-05-24-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | JP-H06141844-A |
| titleOfInvention | Adhesive animal cell culture substrate and culture module |
| abstract | (57) [Summary] [Object] To provide a culture substrate and a culture module for adherent animal cells capable of efficiently producing metabolites. [Structure] A nonwoven fabric composed of high-melting point polyester fibers and low-melting point polyester fibers was washed with distilled water and then heat-sterilized. This was immersed in an atelocollagen aqueous solution, dried, then insolubilized with hexamethylene diisocyanate, and air dried. This culture substrate was set in a flat plate-type bioreactor and subjected to heat sterilization treatment in a phosphate buffer, and then an aqueous solution containing calf serum and glucose as a medium was supplied into the cells of the bioreactor to perform pretreatment. . Then, after removing this medium, a cell inoculating solution containing γCHOAr 2 cells producing human renin was introduced into the cells of the bioreactor to attach the cells to the culture bed. Then, an aqueous solution containing calf serum and glucose was circulated as a medium. |
| isCitedBy | http://rdf.ncbi.nlm.nih.gov/pubchem/patent/WO-2004018615-A1 http://rdf.ncbi.nlm.nih.gov/pubchem/patent/WO-2022163181-A1 |
| priorityDate | 1992-11-13-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 58.