http://rdf.ncbi.nlm.nih.gov/pubchem/patent/JP-H0568554-A
Outgoing Links
| Predicate | Object |
|---|---|
| assignee | http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_1394e66eb648f9bf0a1f5507fe25b67b |
| classificationIPCAdditional | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12R1-885 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12R1-865 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N9-42 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-02 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-08 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N1-19 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N1-15 |
| filingDate | 1991-09-05-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor | http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_2aa33ae822acd8857681b42773aada44 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_bdf0b68af6cf5451212472506fef5d0a |
| publicationDate | 1993-03-23-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | JP-H0568554-A |
| titleOfInvention | Cell fusion method and fused cell obtained by the method |
| abstract | (57) [Summary] [Purpose] Collectively the functions consisting of multiple gene information, It is an object of the present invention to provide a cell fusion method for introducing into a target host cell, particularly a yeast cell, and more specifically to obtain a yeast having cellulase-producing ability using this method. [Constitution] Test cells of fungal conidium or mycelium having a predetermined gene are exposed to a polyploid inducer under non-nutritive conditions to form micronuclei, and then the cells are treated with a cell wall lysing enzyme to form protoplasts. , A yeast is treated with a cell wall lysing enzyme to form a protoplast, and the two protoplasts are fused, so that the gene of a useful product consisting of a plurality of gene groups is mainly incorporated into the micronucleus and other unnecessary The gene group can be prevented from being involved in hybrid formation so much, the frequency of hybrid acquisition in intergeneric protoplast fusion can be improved, and the genetic stability of the resulting intergeneric hybrid can be improved. Further, as the specifically obtained fused cells, TRSC-2, TRSC-3, or TRSC-4 in which Trichoderma reesei having a cellulase-producing gene and Saccharomyces cerevisiae are fused is disclosed. |
| isCitedBy | http://rdf.ncbi.nlm.nih.gov/pubchem/patent/GB-2455481-B http://rdf.ncbi.nlm.nih.gov/pubchem/patent/GB-2455481-A http://rdf.ncbi.nlm.nih.gov/pubchem/patent/WO-2008052759-A1 |
| priorityDate | 1991-09-05-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 104.