| abstract |
(57) [Abstract] [Purpose] From the reading of a region separating relatively large nucleic acid fragments to the reading of a detailed fluorescence pattern for determining each nucleotide sequence using a common nucleic acid electrophoresis pattern reading device. Is performed in series. [Structure] A first step in which nucleic acid of a sample is separated by gel electrophoresis and stained with a staining agent containing ethidium bromide, and a migration pattern of the sample is excited by using a laser beam having a wavelength of 532 nm, The second step of selecting the sample by reading the distribution pattern of the fluorescence intensity generated from the sample in the wavelength region of about 600 nm, and for the selected sample, 532 nm as a fluorescent substance for labeling for base sequence determination The third step is to label with a fluorescent substance that can be excited with a fluorescent substance that emits fluorescence in the vicinity of about 600 nm, perform electrophoresis, and read the fluorescence pattern. |