http://rdf.ncbi.nlm.nih.gov/pubchem/patent/JP-H05292998-A
Outgoing Links
| Predicate | Object |
|---|---|
| assignee | http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_edaeb5e71b74c5e5a48dc2475452145a http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_26c536340aa6d174929c588553cce1db |
| classificationCPCAdditional | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q2326-40 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q2304-26 |
| classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-28 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-04 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-04 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-28 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G01N21-77 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-06 |
| filingDate | 1992-04-17-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor | http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_5a000a6125a2dbbcc66b659696789047 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_c68b6e731bdb7c9a08d04af4914a908f |
| publicationDate | 1993-11-09-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | JP-H05292998-A |
| titleOfInvention | Method for measuring the number of viable cells and vitality |
| abstract | (57) [Abstract] [Purpose] To provide a method capable of conveniently and rapidly measuring the cell activity and the number of viable cells of a large number of samples by using an ordinary microplate reader. [Structure] Oxidized quinone such as 1,4-naphthoquinone, 2-methyl-1,4-naphthoquinone (menadione) or tetramethyl-p-benzoquinone is allowed to act on a measurement system containing living cells to generate hydrogen peroxide. The reaction is performed with an indirect coloring reagent or a direct coloring reagent via an enzyme having hydrogen peroxide as an acceptor, and the color development intensity is quantified with a spectrophotometer (colorimeter). The preferred enzyme is peroxidase, the indirect color reagent is N- (carboxymethylaminocarbonyl) -4,4'-bis (dimethylamino) -diphenylaminosodium) or leuco crystal violet, and the direct color reagent is cytochrome c peroxidase. .. |
| priorityDate | 1992-04-17-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 72.