| abstract |
(57) [Summary] [Structure] A single-stranded nucleic acid probe having a nucleotide sequence complementary to a target gene is prepared by immobilizing a single-stranded denatured sample nucleic acid on the electrode surface or the tip of an optical fiber. Let it react, Further, it specifically binds to the double-stranded nucleic acid and reacts with an electrochemically or photochemically active double-stranded recognizer. After completion of the reaction, an electrochemical or photochemical change derived from the double-stranded nucleic acid is measured via an electrode or an optical fiber. In addition, by labeling the nucleic acid probe with an electrochemically or photochemically active substance and measuring the electrochemical or photochemical change derived from this labeled substance, it is possible to use a double-stranded chain-recognizing substance Gene detection can be performed. [Effect] Genes can be detected safely and easily in a short time without using a radioisotope. |