http://rdf.ncbi.nlm.nih.gov/pubchem/patent/JP-H05245000-A
Outgoing Links
| Predicate | Object |
|---|---|
| assignee | http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_e981a75cc7ccf264e95a0faaf648e684 |
| classificationIPCAdditional | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12R1-32 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G01N33-50 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-68 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-09 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-04 |
| filingDate | 1992-03-06-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor | http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_b5963731fdab3a115cf9acc1ac483886 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_08be597ba0d542ee0c04dffacca2bc52 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_3d7d7a59cea31ec4dffd096bf1f169c1 |
| publicationDate | 1993-09-24-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | JP-H05245000-A |
| titleOfInvention | Specific detection method for Mycobacterium tuberculosis |
| abstract | (57) [Summary] [Objective] To provide a specific, highly sensitive and rapid detection method for Mycobacterium tuberculosis. [Structure] A method for specific detection of a Mycobacterium tuberculosis complex, which comprises extracting from a sample suspected of containing the Mycobacterium tuberculosis complex 16S rR DNA sequence containing the NA gene was extracted, and 16S r A primer complementary to a sequence specific to the M. tuberculosis group in the RNA gene DNA base sequence, which is a combination of an oligonucleotide primer for a sense strand and an oligonucleotide primer for an antisense strand, and 4 kinds of deoxynucleoside triphosphates Acid and DNA A synthase is applied to the extracted DNA solution to amplify a DNA fragment having the nucleotide sequences of both primers at both ends, the amplified DNA fragment is treated with a restriction enzyme, and the DNA after the restriction enzyme treatment The presence of the Mycobacterium tuberculosis group is detected by subjecting the amplified fragment to electrophoresis, immobilizing it on a nucleic acid binding membrane, hybridizing a labeled probe complementary to a part of the DNA amplified fragment, and detecting the label of the labeled probe. And a primer and a probe used in the method. |
| isCitedBy | http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-8186790-B2 http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-7879581-B2 http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-6513894-B1 |
| priorityDate | 1992-03-06-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 62.