| abstract |
(57) [Summary] [Structure] Formula (II) [Chemical Formula 1] [R: H, OH, O- CO-R ";R" :( C 1 ~C 4) A compound having an alkyl group; R 1 : a carboxylic acid group] is selectively deacylated in the presence of a microorganism selected from the genus Pseudomonas, Bacillus, Achromobacter or an enzyme derived therefrom. Formula (I) 7-Amino-cephalosporanic acid with is prepared. [Effect] This method uses a microorganism having high selectivity and high acylase activity, does not require a toxic solvent or reagent, and is performed at a moderate pH and temperature. |