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classificationIPCInventive http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G01N27-414
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filingDate 1992-01-17-04:00^^<http://www.w3.org/2001/XMLSchema#date>
inventor http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_328d877122086c384b3249fb79fb57ac
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publicationDate 1993-08-06-04:00^^<http://www.w3.org/2001/XMLSchema#date>
publicationNumber JP-H05196600-A
titleOfInvention Method for producing immobilized enzyme membrane and protein-immobilized membrane
abstract (57) [Summary] [Object] To provide a protein-immobilized membrane and a method for producing an immobilized enzyme membrane, which have a uniform membrane thickness and can prevent the inactivation of an enzyme due to the contraction of the membrane. [Issue] The immobilized enzyme membrane is a solution 1 to 4 in which a mixed solution of an aqueous protein solution comprising an enzyme and a crosslinking agent contains at least 1 to 20% by weight of enzyme and 10 to 50% by weight of protein. 1 to 2 parts by weight, and 1 to 2 parts by weight of a liquid containing 50 to 100% by weight of a water-soluble crosslinking agent having two or more epoxy groups. It is manufactured by spin-coating a mixed solution consisting of 1 to 2 parts by weight of a solution containing wt% glutaraldehyde on a predetermined part of the surface of the semiconductor field effect ion sensor.
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