http://rdf.ncbi.nlm.nih.gov/pubchem/patent/JP-H0494700-A
Outgoing Links
| Predicate | Object |
|---|---|
| assignee | http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_1374dd16777534b65ad4422333245af8 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-68 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-09 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-10 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-70 |
| filingDate | 1990-08-08-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor | http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_6b6f126030b792ee90f614156970c978 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_50bf2573d87ec93fd2060f88ec3b2b30 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_fea863d86bde7598fdcf4739cc70cb99 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_a5956ffa2d42ccc93dccf9e1d4bb5fd9 |
| publicationDate | 1992-03-26-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | JP-H0494700-A |
| titleOfInvention | High-sensitive detection of c-type hepatitis virus and related nucleic acid and oligo-dna set for primer |
| abstract | PURPOSE: To detect the subject virus for diagnosis of C-type hepatitis in high sensitivity even in an initial stage of affection by synthesizing a cDNA from a nucleic acid prepared from a test substance and amplifying the cDNA polymerase using a specific oligo-DNA set as a primer. n CONSTITUTION: Blood serum of a patient diagnosed as non-A, non-B type chronic hepatitis is ultracentrifuged for 5hr and a resultant pellet is suspended in a buffer solution to prepare a virus suspension, then the suspension is treated with adding proteinase in the presence of a surfactant, thus extracted with phenol/chloroform and an RNA is precipitated with ethanol, then a cDNA is synthesized using said RNA as a mold, thus the cDNA is amplified in treating with DNA polymerase to obtain a DNA group 1 expressed by formula I to formula VI and a DNA group 2 expressed by formula VII to formula XIII. Next, a cDNA is synthesized from a nucleic acid prepared from a test substance and the cDNA is amplified using DNA polymerase and an oligo-DNA set respectively at least a species selected from the DNA group 1 and the DNA group 2 as primers to detect C-type hepatitis virus in high sensitivity. n COPYRIGHT: (C)1992,JPO&Japio |
| priorityDate | 1990-08-08-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 26.