http://rdf.ncbi.nlm.nih.gov/pubchem/patent/JP-H04360686-A
Outgoing Links
| Predicate | Object |
|---|---|
| assignee | http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_6bfdf49224a405be1f7b653c56ebad94 |
| classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-1017 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-10 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-09 |
| filingDate | 1991-06-04-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor | http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_e26bca5411a78a9c16368963f2a1a323 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_98eb668d95167f76fd5e80cbcd3068e2 |
| publicationDate | 1992-12-14-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | JP-H04360686-A |
| titleOfInvention | Purification of dna |
| abstract | PURPOSE: To purify a high-purity DNA in high yield by simple operation without using a harmful reagent by subjecting cells of bacterium to bacteriolysis, passing the cells through a membrane filter to remove insoluble substances, removing impurities by ultrafiltration and concentrating the DNA. n CONSTITUTION: Escherichia coli transformed with a recombinant plasmid (e.g. plasmid pUC119) is cultured in an ampicillin-containing medium at 37°C for 24 hours, a culture solution is centrifuged to collect cells, which are suspended in a tris-hydrochloric acid buffer solution (pH 8) containing 10mMEDTA, subjected to bacteriolysis with 0.2N NaOH-1% sodium dodecyl sulfate, then the mixed solution is filtered under pressure by a membrane filter having 0.1-2μm pore diameter to remove insoluble substances, subjected to ultrafiltration with an ultrafilter having 30,000-1,000,000 fraction molecular weight to remove impurities and concentrated to separate and purify plasmid and/or cosmid DNA from cells of bacterium. n COPYRIGHT: (C)1992,JPO&Japio |
| isCitedBy | http://rdf.ncbi.nlm.nih.gov/pubchem/patent/KR-100390529-B1 http://rdf.ncbi.nlm.nih.gov/pubchem/patent/JP-2006129861-A http://rdf.ncbi.nlm.nih.gov/pubchem/patent/JP-2009189368-A http://rdf.ncbi.nlm.nih.gov/pubchem/patent/WO-9915645-A1 http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-6969603-B2 |
| priorityDate | 1991-06-04-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 27.