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filingDate 1991-01-30-04:00^^<http://www.w3.org/2001/XMLSchema#date>
inventor http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_75247b47bc97ab7ca139625290e19e04
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publicationDate 1992-09-01-04:00^^<http://www.w3.org/2001/XMLSchema#date>
publicationNumber JP-H04244100-A
titleOfInvention Method for immunological measurement of pthrp
abstract PURPOSE: To readily measure the subject substance effective in diagnosis, etc., of hypercalcemia caused by malignant tumor by reacting a specimen with an antibody specific for a parathyroid hormone-related protein and detecting the produced antigen-antibody reaction. n CONSTITUTION: A parathyroid hormone-related protein (PTHrP) expressed by the formula is reacted with Na 125 I using chloramine T to prepare a 125 I- labeled PTHrP. A prescribed amount thereof is then mixed with a specimen such as blood of a patient and the resultant mixture of the antigen with the labeled antigen is subsequently reacted with an antibody specifically recognizing C-terminals of the PTHrP. The produced antigen-antibody complex is then precipitated by centrifugation and separated from the unreacted antigen and antibody. The radiation dose of the formed precipitates is measured with a well type scintillation counter. The measured value is subsequently used to read the concentration from a standard curve prepared by measuring an antigenic standard reagent at well-known concentrations according to a similar method. Thereby, the PTHrP concentration is rapidly and readily determined. n COPYRIGHT: (C)1992,JPO&Japio
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