| abstract |
PURPOSE:To extract a polysaccharide from very small algae without reducing molecular weight of polysaccharide. CONSTITUTION:Very small algae (porphyridium cruentum) are cultured to give a culture solution of algae, from which wet algae (13wt.% solid content) are obtained by centrifugal separation, 1kg of the wet algae are incorporated with 2 liter water and adjusted to pH 9 with 1 N-NaOH. Then, the blend is adjusted to pH 7 with 1N-HC1, subjected to enzymatic reaction with 13g protease (papain) at 50 deg.C for 24 hours, mixed with an equal amount of ethanol and a polysaccharide is floated and recovered. The recovered polysaccharide is dissolved in water, further sufficiently subjected to enzymatic reaction with 13g papain at 50 deg.C for 24 hours and mixed with ethanol and the polysaccharide is floated and recovered. |