http://rdf.ncbi.nlm.nih.gov/pubchem/patent/JP-H04200400-A
Outgoing Links
| Predicate | Object |
|---|---|
| assignee | http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_be3928f66bb2545a8175c61c56146b9a |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-70 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-68 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-09 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-10 |
| filingDate | 1990-11-30-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor | http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_13d416a6e8b1994c9021e21f5ecfa8fe http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_8c75973a86bdb2ec7646a6e3f172e564 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_65cf759cc3b28c631102e700eacea1c6 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_81b26df6f8cf8c69463d28ac6b65f164 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_fe0ad382b8be749845770d661b03656a http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_eae2e41508e9a36bda57319ab2996279 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_175756b67eebbce75d0413c6ebd947af |
| publicationDate | 1992-07-21-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | JP-H04200400-A |
| titleOfInvention | Extraction of virus genom specimen originated human body and detection of the virus genom |
| abstract | PURPOSE: To enable easy, quick and accurate detection of a virus genom by treating a bio-specimen with a proteinase in the presence of a nonionic surfactant. n CONSTITUTION: A bio-specimen such as 1-20μl of the serum or plasma of viral infection patient or ≤10mg of an extracted tissue is mixed with a nonionic surfactant (e.g. Nonidet P40) and a proteinase (e.g. Proteinase K) and reacted to obtain a virus genom (A) such as human hepatitis C virus genom. When the component A is DNA (B), the component is used as it is and when the component A is RNA (C), the component C is converted into a DNA (D) prior to the following treatment. A liquid containing a primer having the base sequence of formula I, a primer having the base sequence of formula II and Taq polymerase is added to the component A and subjected to the 1st polymerase chain reaction (PCR) to obtain a reaction product (E). The component E is added with the primers of formula III and formula IV, etc., to obtain the 2nd PCR reaction product (F). The component F or the component B is subjected to electrophoresis and the band corresponding to the desired base pair length is examined to detect the objective virus genom. n COPYRIGHT: (C)1992,JPO&Japio |
| isCitedBy | http://rdf.ncbi.nlm.nih.gov/pubchem/patent/JP-H04267899-A http://rdf.ncbi.nlm.nih.gov/pubchem/patent/JP-H0751080-B2 http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-5730971-A |
| priorityDate | 1990-11-30-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 29.