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classificationIPCInventive http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G01N33-92
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G01N21-76
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G01N33-50
filingDate 1990-11-01-04:00^^<http://www.w3.org/2001/XMLSchema#date>
inventor http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_f78e5a9c6c48473bac6556c6fc8ae0bb
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publicationDate 1992-06-17-04:00^^<http://www.w3.org/2001/XMLSchema#date>
publicationNumber JP-H04169848-A
titleOfInvention Method for measuring superoxide anion radical derived from blood or constituent component thereof
abstract PURPOSE: To make it possible to measure superoxide anion radical directly and sensitively by using blood and its constituent component as an object to be measured and Cypridina luciferin or its derivative as luminescence reagent. n CONSTITUTION: At first, blood or its constituent component as an object to be measured is fractionated. The constituent component of a cell such as erythrocyte is used by breaking the cell. The cell breaking is performed by inputting the fraction cell into hypotonic solution represented by, e.g. water, Tris buffer or the like. Thereafter, Cyprina luciferin or its derivative is used as luminescence reagent for the solution obtained by the cell breakdown. The amount of the superoxide anion radial is measured by chemiluminescence. It is preferable that the cell breaking operation is made to progress at the same time of the addition of the luminescence reagent. The luminescence reagent is the derivative of the Cypridina luciferin, and it is preferable to use 2-methyl-6-phenyl-3 or the like. Thus, the superoxide anion radical can be directly measured sensitively. n COPYRIGHT: (C)1992,JPO&Japio
isCitedBy http://rdf.ncbi.nlm.nih.gov/pubchem/patent/JP-2017513001-A
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Total number of triples: 26.