| abstract |
NEW MATERIAL:A peptide having a skeleton of formula Trp-His-His-Trh. USE:A low toxic angiotensinase inhibitor useful as a preventive and therapeutic agent of hypertension, an antihypertensive agent, a therapeutic agent of congestive cardiac failure, etc., with high safety. PREPARATION:A protein (preferably fish protein such as dried bonito or actin) is initially mixed with (hot) water and the resultant mixture is homogenized by a strong stirring force. Thermolysin is then added thereto followed by hydrolysis at 10-85 deg.C for 0.1-48hr. The resultant hydrolyzed solution is concentrated by centrifugal separation and subsequently purified by high performance liquid chromatography, etc. |