http://rdf.ncbi.nlm.nih.gov/pubchem/patent/JP-H0413697-A

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http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C07K14-435
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filingDate 1990-04-28-04:00^^<http://www.w3.org/2001/XMLSchema#date>
inventor http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_de8c381a03840d9297768b9e391f4cf5
publicationDate 1992-01-17-04:00^^<http://www.w3.org/2001/XMLSchema#date>
publicationNumber JP-H0413697-A
titleOfInvention Bioactive peptide
abstract NEW MATERIAL:A bioactive peptide having following physicochemical properties. molecular weight: about 10400 measured by SDS electrophoresis and about 10900 measured by HPLC, isoelectric point: 7.12, bioactivity: having chymotrypsin-inhibiting activity. n USE: Used as a chymotrypsin inhibitor having pH-stability and heatstability. n PREPARATION: For instance, blood cell is removed from blood in larva of domesticated silkworm Bombyx mori i10 by centrifuge and ammonium sulfate is added to blood plasma of the resultant blood, then precipitate precipitated at 70% saturation is centrifuged, thus resultant precipitate is dissolved in citric acid buffer solution (pH4.5) and resultant buffer solution is dialyzed, then subjected to cation-exchange column chromatography, thus eluted by 0-0.5M linear concentration gradient of NaCl and active fraction is purified by subjecting to affinity column chromatography using chymotrypsin-combining column to afford the aimed bioactive peptide having chymotrypsin-inhibiting activity. n COPYRIGHT: (C)1992,JPO&Japio
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Total number of triples: 32.