http://rdf.ncbi.nlm.nih.gov/pubchem/patent/JP-H04126092-A
Outgoing Links
Predicate | Object |
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assignee | http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_8e43b4ca6b41ce9f54c9a895d474c81a |
classificationCPCAdditional | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Y115-01001 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Y111-01006 |
classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N9-0065 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N9-0089 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C07K1-20 |
classificationIPCAdditional | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12R1-19 |
classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C07K14-005 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N1-21 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C07K14-195 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C07K14-41 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C07K1-20 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N9-02 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12P21-00 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C07K1-16 |
filingDate | 1990-09-14-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
inventor | http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_ec5452f2eee8e31cdf29e4aef81adaa6 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_f0a6f0be0b2c14cb1ec857a746c72012 |
publicationDate | 1992-04-27-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
publicationNumber | JP-H04126092-A |
titleOfInvention | Removing of protein derived from escherichia coli |
abstract | PURPOSE: To efficiently remove a protein derived from Escherichia coli by bringing an aqueous solution containing the protein derived from Escherichia coli into contact with a phenyl group-bonding resin. n CONSTITUTION: A gene-recombination process is performed using Escherichia coli as a host to obtain an aqueous solution (C) of protein containing a protein (A) derived from Escherichia coli and a gene-recombinant protein (B). Next, a solution of the component C containing 0.2-2.0 M (NH 4 ) 2 SO 4 , etc., is pressed through a phenyl group-bonding resin (E) equilibrated with a buffer solution (D) of citric acid-based, etc., at pH 4-10 to make the component A adsorbed to the component E. Then, the component D is flowed in an amount of 3-5 times of the component E to wash the component E, thus the component B is recovered from by passed fraction. Next, (NH 4 ) 2 SO 4 solution having reduced concentration is passed through the component E to which the component A is adsorbed, then the component A is eluted to remove the protein derived from Escherichia coli. n COPYRIGHT: (C)1992,JPO&Japio |
priorityDate | 1990-09-14-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 31.