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filingDate 1990-09-17-04:00^^<http://www.w3.org/2001/XMLSchema#date>
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publicationDate 1992-04-27-04:00^^<http://www.w3.org/2001/XMLSchema#date>
publicationNumber JP-H04126091-A
titleOfInvention Production of quercetin glucuronide and cultured cell containing the same
abstract PURPOSE: To make quercetin glucuronide to be safely and widely utilized by extracting a specific cultured cell. n CONSTITUTION: A leaf of Euphorbia millii as a plant belonging to genus Euphorbia is washed with deionized water and extracted by alcohol, etc., then cut, thus the resultant small piece is cultured in a medium containing auxin-affecting substance, etc., by light irradiation to obtain cultured cell (B) as callus, differentiated tissue, cultured organ, cultured root or cultured foliage abundantly containing quercetin glucuronide (A). Next, the component B is freeze-dried and extracted with a neutral organic solvent (C) such as butanol. to obtain an extracted solution (D). Then, the component D is filtered and resultant filtered solution is concentrated under reduced pressure below 40°C, then washed with ether, etc., thus extracted with butanol, etc., and separated with liquid chromatography, etc., then purified to produce the aimed quercetin glucuronide. n COPYRIGHT: (C)1992,JPO&Japio
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