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filingDate 1989-12-20-04:00^^<http://www.w3.org/2001/XMLSchema#date>
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publicationDate 1991-01-14-04:00^^<http://www.w3.org/2001/XMLSchema#date>
publicationNumber JP-H037590-A
titleOfInvention Production of l-alanine
abstract PURPOSE: To efficiently obtain L-alanine in high yield by blending and reacting fumaric acid, etc., with ammonia, etc., and α-keto acid in the presence of a specific cell in a water-soluble solvent. n CONSTITUTION: A strain of coryneform bacterium is aerobically cultured in a glucose medium, etc., at 20-40°C at pH5-10 for 2-9 days to give an aspartase- containing bacterium cell (treated material) (A). Then a strain of the genus Pseudomonas is aerobically cultured in a medium containing fumaric acid, etc., at 20-40°C at pH5-10 for 1-3 days to give an aspartic acid β-decarboxylase- containing bacterium cell (treated material) (B). Then the components A and B, 0.5-30wt.% fumaric acid (salt), 0.0001-0.5wt.% pyruvic acid (salt) or α-keto acid such as α-ketobutyric acid (salt) and 0.1-5mol ammonium (ion) are added to an aqueous solution, optionally mixed with 0.0005-0.05wt.% pyridoxal-5'- phosphate and reacted at pH6-10 at 40-50°C for 5-72 hours. Then the prepared reaction solution is purified by an ion exchange resin to produce L-alanine. n COPYRIGHT: (C)1991,JPO&Japio
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http://rdf.ncbi.nlm.nih.gov/pubchem/patent/JP-2012026524-A
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priorityDate 1989-03-16-04:00^^<http://www.w3.org/2001/XMLSchema#date>
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