| abstract |
PURPOSE:To produce mucin useful as a drug in high efficiency by transforming a host cell with a vector containing a DNA coding a mucin [a fibroblast cell growth factor(FGF)] having one or more sugar-chain bonding sites introduced into the mucin. CONSTITUTION:A host cell such as yeast or animal cell is transformed with a vector containing a DNA coding a mucin (a fibroblast cell growth factor) having one or more sugar-chain bonding sites introduced therein. The transformed host cell is cultured in a medium to produce and accumulate the mucin in the cultured product, from which mucin is recovered. The mucin- constituting sugar chain is preferably N-acetylglucosamine, N- acetylgalactosamine, mannose, galactose, sialic acid, etc. |