http://rdf.ncbi.nlm.nih.gov/pubchem/patent/JP-H0339019-A

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http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/A01C1-06
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/A01H4-00
filingDate 1990-05-17-04:00^^<http://www.w3.org/2001/XMLSchema#date>
inventor http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_a7ba859e2f1e8708e65fb3421545c48a
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publicationDate 1991-02-20-04:00^^<http://www.w3.org/2001/XMLSchema#date>
publicationNumber JP-H0339019-A
titleOfInvention Storing method of vegetable germ
abstract PURPOSE: To prevent generation of secondary adventive germ or formation of callus by preliminarily treating vegetable germ in a culture medium containing an increasing agent for osmotic pressure and freezing the vegetable germ at specified temp. n CONSTITUTION: Vegetable germ is inoculated in a Murashige culture medium and Skoog culture medium containing an increasing agent for osmotic pressure (such as sucrose) by 75 to 190gL -1 and preliminarily treated at 18 to 24°C for 30sec to 36 hours with 150 to 250 lux illumination. Then the vegetable germ is frozen by cooling at 0.1 to 1°C/min cooling rate to -15 to -40°C. The frozen germ is defrosted, washed to remove the culture medium and inoculated in a Murashige and Skoog culture medium to grow. n COPYRIGHT: (C)1991,JPO
priorityDate 1989-05-23-04:00^^<http://www.w3.org/2001/XMLSchema#date>
type http://data.epo.org/linked-data/def/patent/Publication

Incoming Links

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Total number of triples: 19.