| abstract |
PURPOSE: To prepare a glycoside having physiological activity and physical properties important for drugs, etc., by treating an aglycon with a specific saccharase obtained from the genus Stevia plant. n CONSTITUTION: An aglycon is treated with a saccharase (I) or (II) to provide the objective glycoside. The saccharase (I) produces steviolmonoside when steviol is used as an aglycon and also produces stevioldioside when the steviolmonoside is used as an aglycon. The saccharase (II) produces stevioside when the stevioldioside is used as an aglycon and also produces rebaudiside A when the stevioxide is used as an aglycon. The extraction of the enzymes from the genus Stevia plant is performed e.g. by adding a 2-10 times volume of a buffer solution to the raw leaves of the plant, grinding the mixture with a mixer, etc., for several minuted, subjecting the ground mixture to filtration and centrifugal separation processes and subsequently subjecting the prepared transparent enzyme solution to ammonium sulfate, dialysis, gel filtration and column chromatographic treatments, etc. n COPYRIGHT: (C)1991,JPO&Japio |