http://rdf.ncbi.nlm.nih.gov/pubchem/patent/JP-H03266981-A
Outgoing Links
| Predicate | Object |
|---|---|
| assignee | http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_3a4c2aa04b5d2999f6f4f50f08987255 http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_69ac1d45776a8a24b24fec258548e537 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N5-07 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N5-071 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N5-10 |
| filingDate | 1990-03-16-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor | http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_2f33b570038c6ec49b435ab84085c5f4 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_ed984b915997e8862c72e06858c5a933 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_09c9ecf81088ccd2ee5ef549bedf3df5 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_fdb64b27161e15821db241844fc7e94f http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_aeefd93d92643e9f0d50d3123ef2a894 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_663f412f6ccfb8dcaf24e63ce4ccdfad |
| publicationDate | 1991-11-27-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | JP-H03266981-A |
| titleOfInvention | Synthetic medium for animal cell |
| abstract | PURPOSE: To multiply cells and to obtain the same amount of physiologically active substance as that in a conventional way even by addition of low concentration serum of about 1 %, by adding specific culture additives such as iron sulfate or succinic acid to an alpha MEM base medium. n CONSTITUTION: Ferrous sulfate heptahydrate, N-acetyl-D-glucasamine, succinic acid, glutathione (reduction type) and putrescine dihydrochloride are added to an alpha EME medium as a base medium, further enriched with choline chloride and i-inositol as vitamins in composition of the alpha MEM medium and concentration of ascorbic acid is reduced to give a synthetic medium. when animal cells such as CHO-KI or BHK are cultured by using the synthetic medium, multiplication of animal cells and production of physiologically active substance are promoted. Consequently, even by addition of low concentration of serum of about 1%, multiplication of cells and the amount of physiologically active substance produced by cells can result in the same degree or more than the conventional method in comparison with alpha EME medium containing about 5-10% serum used in the conventional method. n COPYRIGHT: (C)1991,JPO&Japio |
| isCitedBy | http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-109988741-A http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-105176916-A |
| priorityDate | 1990-03-16-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 44.