| abstract |
PURPOSE: To shorten reaction time for cutting out saccharide chains for analyzing a glycoprotein useful as a medicine and diagnostic agent by reacting the glycoprotein in contact with hydrazine vapor. n CONSTITUTION: A glycoprotein is initially dried in a small tube and the tube containing the aforementioned glycoprotein therein is then installed in a hermetically sealable reaction vessel. The interior of the reaction vessel is preferably replaced with nitrogen, etc., and subsequently decompressed with preferably a vacuum pump. Hydrazine is further injected thereinto and preferably reacted at 130°C for 30min to 1hr. After the reaction, the above-mentioned tube is taken out and toluene is added thereto. The resultant mixture is then subjected to vacuum evaporation to dryness. The aforementioned operation, as necessary, is repeated once more to cut out the objective saccharide chains. n COPYRIGHT: (C)1991,JPO&Japio |