http://rdf.ncbi.nlm.nih.gov/pubchem/patent/JP-H03178996-A

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filingDate 1989-12-06-04:00^^<http://www.w3.org/2001/XMLSchema#date>
inventor http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_2cb01695dbcb5593b359d84edaab88d9
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publicationDate 1991-08-02-04:00^^<http://www.w3.org/2001/XMLSchema#date>
publicationNumber JP-H03178996-A
titleOfInvention Growth factor
abstract NEW MATERIAL:A growth factor contained in the culture supernatant of a fibroblast-like cell originated from chronic rheumatism synovial cell and having the following properties. The mol.wt.: 50000-70000, not having affinity to heparin, stable on thermal treatment at 56°C for 30min and promoting the growth of vascular endothelial cell. n USE: A treatment-promoting drug for burns and wounds, a remedy for angiopathy, or a remedy or diagnostic agent for chronic rheumatism and solid tumors. n PREPARATION: For example, synovial cell is collected from the knee arthrosis of a chronic rheumatism patient, finely cut and treated with trypsin in a buffer solution. Cells contained in the supernatant are collected, cultured in a medium and cloned by a limiting dilution analysis. The fibroblast-like cells are cultured and the supernatant of the cultured solution is concentrated and purified by a high performance liquid chromatography to provide the growth factor having a mol.wt. of 50000-70000. n COPYRIGHT: (C)1991,JPO&Japio
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