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filingDate 1989-07-12-04:00^^<http://www.w3.org/2001/XMLSchema#date>
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publicationDate 1991-01-23-04:00^^<http://www.w3.org/2001/XMLSchema#date>
publicationNumber JP-H0315397-A
titleOfInvention Production of glucosyl glycyrrhetinate and anti-inflammatory agent
abstract PURPOSE: To enable advantageous production of β-D-glucosyl glycyrrhetinate and utilization thereof by culturing a cell of a plant belonging to the genus Eucalyptus in a culture medium containing glycyrrhetinic acid or a salt thereof. n CONSTITUTION: A section of young tissues of a plant belonging to the genus Eucalyptus, such as Eucalyptus perrioina, is taken from a part, such as stem or leaf, sterilized, then cut into a suitable size and planted to a culture medium prepared by adding a carbon source, such as sucrose, and a phytohormone, such as auxin, to a Murashige-Skoog culture medium, etc., to induce calli. The resultant calli are subsequently inoculated into a liquid culture medium and subjected to shaking culture. After the number of the cells attains the maximum, a solution of glycyrrhetinic acid or salt thereof in ethanol is added to subsequently continue the culture. The obtained culture solution is then separated and purified to isolate β-D-glucosyl glycyrrhetinate expected to be extensively utilized as an anti-inflammatory agent in the form of a skin liniment or oral administration agent. n COPYRIGHT: (C)1991,JPO&Japio
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