http://rdf.ncbi.nlm.nih.gov/pubchem/patent/JP-H03133376-A

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http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N1-20
filingDate 1989-10-18-04:00^^<http://www.w3.org/2001/XMLSchema#date>
inventor http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_3d605e3bc3d4e2c387b17e2f087cf110
publicationDate 1991-06-06-04:00^^<http://www.w3.org/2001/XMLSchema#date>
publicationNumber JP-H03133376-A
titleOfInvention Production of sarcosine oxidase
abstract PURPOSE: To obtain sarcosine oxidase extremely useful as an enzyme for determining creatinine or creatine in blood and urine at a practical level by culturing UTK3044 strain belonging to the genus Bacillus in a synthetic medium or natural medium. n CONSTITUTION: UTK3044 strain (FERM P-10849) is cultured in a medium capable of growing the strain, namely a synthetic medium or natural medium containing a carbon source, a nitrogen source, an inorganic salt and another nutrient. The culture temperature is usually 30-50°C, preferably about 40°C. pH of the culture solution is 6.5-8.0. Shaking culture or aerated spinner culture is carried out under the above-mentioned condition for 5-18 hours to efficiently produce sarcosine oxidase (SAO) in the culture mixture with growth of the bacteria. The SAO has physical and chemical properties shown by table 1. n COPYRIGHT: (C)1991,JPO&Japio
isCitedBy http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-109957553-A
priorityDate 1989-10-18-04:00^^<http://www.w3.org/2001/XMLSchema#date>
type http://data.epo.org/linked-data/def/patent/Publication

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