http://rdf.ncbi.nlm.nih.gov/pubchem/patent/JP-H0292299-A
Outgoing Links
| Predicate | Object |
|---|---|
| assignee | http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_983fa366d54b080542742d72951c002c |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G01N33-535 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-28 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G01N33-50 |
| filingDate | 1988-09-30-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor | http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_d3d6b8f3cad9fe7d3fe9678b4a74ed37 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_9437cebfc227c8b8810f4ca96eb49ba5 |
| publicationDate | 1990-04-03-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | JP-H0292299-A |
| titleOfInvention | Measurement using enzyme peroxide |
| abstract | PURPOSE: To perform measurement of activity of enzyme peroxide with high sensitivity by using H 2 O 2 , 3-methyl-2-benzothiazolinone hydrazonate (MBTH) and arylalcohol derivative and measuring said activity in pH range near neutral. n CONSTITUTION: A solution containing enzyme peroxide or a solid phase containing enzyme peroxide in enzyme immunoassay is reacted with a solution containing H 2 O 2 , MBTH and arylalcohol derivative and activity of said enzyme is measured. As an essential condition, said measurement is performed within a range of pH6.9-7.2. MBTH may be hydrochloric acid or other water-soluble salt such as sulfate and a compound expressed by formula I or II is used as arylalcohol derivative. In the measurement, phosphoric acid buffer solution is preferably used, reaction temperature is usually in a range of room temperature - 37°C, dimethylsulfoxide, etc., may be used as reaction stopping solution and absorbance may be measured by a spectrophotometer using a reaction buffer solution not containing enzyme peroxide as a reference after stopping of the reaction. n COPYRIGHT: (C)1990,JPO&Japio |
| priorityDate | 1988-09-30-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 24.