abstract |
PURPOSE: To obtain high-purity phosphatidylserine through a safe, simple process by reaction between phosphatidylcholine and serine using phospholipase D stemmed from microorganisms classified as Streptomyces. n CONSTITUTION: For example, using a reactor equipped with an agitator and temperature controller, each specified amount of (A) calcium chloride, serine and phospholipase D stemmed from microorganisms classified as Streptomyces is added to (B) an aqueous acetic acid- or phosphoric acid buffer solution to effect dissolution. Thence, phosphatidylcholine is dissolved in e.g., ethyl acetate or ether, and a specified amount of the resultant solution is added to the above- mentioned aqueous solution followed by keeping the resultant system at 25-35°C which is agitated and made to react, thus obtaining the objective high-purity phosphatidylserine. n COPYRIGHT: (C)1990,JPO&Japio |