| abstract |
NEW MATERIAL:A peptide (salt) expressed by the formula ((1) and (2) and (3)and (4) are respectively directly bonded; Arg-NH 2 indicates that carboxyl group arginine is amide). n USE: A medical antimicrobial agent, preservative or coating preservative. n PREPARATION: For example, hematocytes of Limulus polyphemus that is a product of North America are added to a buffer solution at pH8.0, pulverized and centrifuged to collect precipitates, which are then added into 20mM hydrochloric acid, repulverized and centrifuged to provide a supernatant. The resultant supernatant is subsequently freeze-dried, redissolved, then subjected to column chromatography and eluted with 0.1% trifluoroacetic acid with acetonitrile concentration changed to 22-30%. The obtained column is then eluted with 28-30% acetonitrile and the eluted fraction is freeze-dried to afford polyphemusin I expressed by the formula. n COPYRIGHT: (C)1990,JPO&Japio |