| abstract |
PURPOSE: To obtain an organ-storing device capable of surely judging the storage state by providing with a means for feeding a perfusate containing marked adenosine triphosphate into a circuit, a means for sampling the perfusate after passed through the organ and means for measuring the concentration of the adenosine triphosphate in the used perfusate. n CONSTITUTION: A perfusate 9 containing marked adenosine triphosphate(ATP) is flown in a closed circuit continuing from a reservoir 8 to a pump 2, a bubble trap 3 and an organ 4 in a storing chamber 5. When the storage state of the organ 4 is measured, the first three-way stopcock 6 and the second stopcock 10 are actuated to flow the perfusate 9a containing a specified concentration of fluorescent ATP from a tank 25 into the first branching way 7 through the third branching way 11, the pump 2 and the organ 4. The concentration of the fluorescent ATP is measured between the bubble trap 3 and the storing chamber 5 and the concentration of the fluorescent ATP after passed the organ is also measured with a cell for measuring the fluorescence, thereby knowing the storage state of the organ 4 from the measured results on the decrease degree of the ATP. The device permits to surely judge the storage state of the organ without relating to a difference between individual organs. n COPYRIGHT: (C)1990,JPO&Japio |