http://rdf.ncbi.nlm.nih.gov/pubchem/patent/JP-H02276574-A
Outgoing Links
| Predicate | Object |
|---|---|
| assignee | http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_c9375aa5bcbe9afbf7152564fd27387c |
| classificationIPCAdditional | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12R1-91 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N9-08 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N5-04 |
| filingDate | 1989-04-17-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor | http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_2218580fb6b3525db13247791ac85962 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_1ed96c8a8d8798541f54125355bf9f55 |
| publicationDate | 1990-11-13-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | JP-H02276574-A |
| titleOfInvention | Production of peroxidase with cultured cell of wasabia japonica matsum. and method for purifying peroxidase from culture |
| abstract | PURPOSE: To stably improve production efficiency at a low cost by culturing tissual pieces or cell pieces separated from a plant of the genus Wasabia in an artificial nutrient culture medium. n CONSTITUTION: A cell strain capable of highly producing a peroxidase is selected from a plant of the genus Wasabia, such as Wasabia japonica Matsum. (Japanese horseradish) or wasabia tenuis Matsum., and protoplasts separated from leaves of the above-mentioned strain are then regrown to provide tissual pieces or cells (A). The resultant cells (A) are subsequently cultured in an artificial nutrient culture medium containing sucrose, auxin, etc., by a shaking culture method, etc., to afford a culture (B). The obtained ingredient (B) is then passed through a concanavalin A-Sepharose(R) column, adsorbed thereon and eluted with methyl-α-D-mannoside to provide an eluate (C). The obtained ingredient (C) is subsequently purified by salting out with ammonium sulfate, etc., to produce the high-purity peroxidase. n COPYRIGHT: (C)1990,JPO&Japio |
| priorityDate | 1989-04-17-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 28.