http://rdf.ncbi.nlm.nih.gov/pubchem/patent/JP-H02234698-A
Outgoing Links
| Predicate | Object |
|---|---|
| assignee | http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_ad2cd796f8c4dcb425ddcc68707d3444 |
| classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N33-581 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-32 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-66 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G01N33-58 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-42 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-32 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-68 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-66 |
| filingDate | 1989-03-08-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor | http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_a4f8589bf1c930b9fa3ff1020c296a6a http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_3a6d2fb28524bcfddc532e5ce7c0cb56 |
| publicationDate | 1990-09-17-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | JP-H02234698-A |
| titleOfInvention | Detecting method of dna probe by bacterium-luciferase system |
| abstract | PURPOSE: To make possible to measure DNA probe with high sensitivity to light and high stability by bonding DNA probe labelled with alkali phosphatase to light-emitting system in specific method. n CONSTITUTION: In fixing specific sequence of nucleic acid, DNA probe labelled with alkali phosphatese is reacted with nicotinamideadenine dinucleotide phosphate (NADP + ) to generate nicotinamideadenine dinucleotide (NAD + ). Said NAD + is reacted with alcohol and alcohol-dehydrogenating enzyme to transform reduction type NAD (NADH). Next, said NADH is affected by flavin reductase and luciferase obtained from luminous bacterium to measure height of emitting peak, then existing amount of alkali phosphatase, amount of enzyme-labelled DNA probe and further amount of nucleic acid sequence formed hybrid with said DNA probe are known. n COPYRIGHT: (C)1990,JPO&Japio |
| priorityDate | 1989-03-08-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 32.