http://rdf.ncbi.nlm.nih.gov/pubchem/patent/JP-H02200200-A
Outgoing Links
| Predicate | Object |
|---|---|
| assignee | http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_7f62824da9d41b863ecaed02e6fd87d2 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-26 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-32 |
| filingDate | 1989-10-05-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor | http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_f2c5d0686ae2eef3c5056028529c3c34 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_ce8269c3025e8c6663086256d16ee272 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_5822925c9f02865a1abc549154fdbacd |
| publicationDate | 1990-08-08-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | JP-H02200200-A |
| titleOfInvention | Determining method of nadh and determination of bile acid using the same method |
| abstract | PURPOSE: To make possible to measure in high accuracy by reacting reduction- type β-nicotinamide adenine dinucleotide(NADH) with oxidation-type glutathione in the presence of specific enzyme. n CONSTITUTION: Oxidation-type glutathione, glutathione.reductase and pH5.5-8 buffer solution containing disulfide-type thiol determining reagent of 5,5'-dithiobis(2-nitrobenzoic acid) are added to a sample containing NADH and reacted at about 37°C for 5-20 min. Next, oxidation-type β-nicotinamide adenine dinucleotide (NAD + ) and reduction-type glutathione are generated in a reacting solution and thiol compound is conjugately generated from the determining reagent. Then, light absorbancy at 300-450nm as characteristic wavelength of the thiol compound is measured to determine NADH. Bile acid is reacted with NAD + in the presence of 3α-hydroxysteroid.dehydrogenase at pH5.5-8, as necessary, and bile acid is determined according to generated amount of NADH. n COPYRIGHT: (C)1990,JPO&Japio |
| isCitedBy | http://rdf.ncbi.nlm.nih.gov/pubchem/patent/JP-2017534882-A http://rdf.ncbi.nlm.nih.gov/pubchem/patent/WO-2018216757-A1 http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-10488407-B2 http://rdf.ncbi.nlm.nih.gov/pubchem/patent/JP-WO2018216757-A1 |
| priorityDate | 1988-10-07-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 87.