http://rdf.ncbi.nlm.nih.gov/pubchem/patent/JP-H02134323-A
Outgoing Links
| Predicate | Object |
|---|---|
| assignee | http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_02ff914937eb6ceaee5ca69cc56cbfa7 http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_298247d28576d4b34507c1b3e6ed893a |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C07K14-52 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C07K1-14 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C07K14-435 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C07K14-47 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/A61P1-16 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/A61K35-26 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/A61K35-28 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12P21-00 |
| filingDate | 1988-11-15-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor | http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_bb5ee54d8576515cc22095ec27367af3 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_a73cb701715eded06da66cb244dc4b95 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_5ffa9dfeb8a9b6a5c3e22e229c9229ed http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_a92975a383ec9cd6a5d4691c609d05a0 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_feae4e6db1adf3d00e496ff01d806927 |
| publicationDate | 1990-05-23-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | JP-H02134323-A |
| titleOfInvention | Production of hepatocyte-proliferation factor |
| abstract | PURPOSE: To provide the subject factor extracted and separated from intercellular substance of spleen tissue and having activity to promote the proliferation of hepatocyte in vitro. n CONSTITUTION: Fresh spleen is extracted from a slaughtered animal such as rat, cattle or pig, placed on a mesh of e.g., stainless steel and crushed to loosen the cell cluster. The cells are washed with a proper amount of a physiological salt solution such as physiological salt solution buffered with phosphoric acid to collect the intercellular substance in a container placed under the mesh. The obtained intercellular substance is centrifuged and a soluble fraction containing the hepatocyte-proliferation promoting factor is obtained as the supernatant liquid. The proliferation factor has the following characteristics. (i) Molecular weight is 40,000 and 150,000 or more. (ii) It is not adsorbed to a heparin column. (iii) The hepatocyte-proliferation promoting activity is inactivated by the heat-treatment at 100°C for 2min. (iv) The hepatocyte-proliferation promoting activity is not inactivated in the presence of trypsin (treated at 34°C for 2hr at a concentration of 0.01%). (v) It has an activity to effect the proliferation of hepatocyte in vitro. n COPYRIGHT: (C)1990,JPO&Japio |
| isCitedBy | http://rdf.ncbi.nlm.nih.gov/pubchem/patent/WO-9308821-A1 http://rdf.ncbi.nlm.nih.gov/pubchem/patent/WO-9306853-A1 |
| priorityDate | 1988-11-15-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 54.