| abstract |
NEW MATERIAL:A protein derived from Canoderma mycelium, not coagulating human erythrocyte, having immunosuppressive ability and amino acid sequence of primary structure part expressed by the formula, etc. USE:An immunosuppressive agent. PREPARATION:For instance, Canoderma mycelium (FERM-P1826) is inoculated in medium and cultured, then cultured solution is centrifuged and mycelium is collected, thus the mycelium is suspended in buffer solution and pulverized, centrifuged, then supernatant is collected and extracted solution is obtained. Next, said extracted solution is subjected to gel chromatography and fractionated, then the active fraction is subjected to ion-exchange chromatography and adsorbed, eluted with 10mM tris-hydrochloric acid buffer solution of pH8.0, pooled the active fraction, dialyzed and freeze-dried to afford the protein expressed by the formula, etc. |