| abstract |
PURPOSE:To measure human arginase, in human blood serum, etc., by synthesizing an antiarginase monoclonal antibody capable of binding to human arginase using an arginase of a mammal other than humans as an antigen. CONSTITUTION:A splenic cell obtained from a mouse immunized with purified or crude arginase is mixed and fused to a myelomatous cell of the same strain mouse in the presence of a cell fusion agent, such as polyethylene glycol, and then cultivated. The resultant hybridoma is selectively propagated using a hypoxanthine-aminopterin-thymidine culture medium, etc., to select cells capable of producing an antiarginase antibody. The obtained cells are subsequently propagated in an ordinary liquid culture medium, etc., to provide an antiarginase monoclonal antibody, which is then used to immunochemically measure a human arginase. |