http://rdf.ncbi.nlm.nih.gov/pubchem/patent/JP-H01265897-A
Outgoing Links
| Predicate | Object |
|---|---|
| assignee | http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_4a5c28b7b2b27f6819b49ca11dcc651a |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-34 |
| filingDate | 1988-04-15-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor | http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_65f80a63601bc69cdffdf87cd9f59fdb |
| publicationDate | 1989-10-23-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | JP-H01265897-A |
| titleOfInvention | Method for determining enzyme |
| abstract | PURPOSE: To precisely and inexpensively determining an enzyme in a short time, by determining strength of fluorescence of 4-methylumbelliferone obtained by hydrolyzing a specific substrate with N-acetyl-β-D-glucosaminidase. n CONSTITUTION: 4-Methylumbelliferyl-N-acetyl-β-D-glucosaminido (4MU) is blended with citric acid to give a substrate solution (A) having pH 4.3-4.9. Then an enzyme-inactivated skim milk collected from a normal cow is added to defatted product of a milk affected by mastitis and reacted with a definite amount of N-acetyl-β-glucosaminidase (NAG) to provide a standard solution (B). Then, fluorescence strength (C) of 4-methylumbelliferone obtained by adding NAG to the liquid A and subjecting the liquid A to hydrolysis reaction and fluorescence strength obtained by reacting the liquid A with a specimen sample are subjected to comparative determination to measure an enzyme such as NAG of cow milk, etc. n COPYRIGHT: (C)1989,JPO&Japio |
| isCitedBy | http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-103484525-A |
| priorityDate | 1988-04-15-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 21.